PureHMO® Human Milk Oligosaccharide Super Prebiotic Powder



Human milk oligosaccharides (HMOs) are a super prebiotic occurring naturally in mother's milk. Today, through precise fermentation, bio-identical HMO delivers all the benefits without any actual human milk in the product. PureHMO® Prebiotic Powder contains 45 one-scoop servings of 1950mg each. 

  • Promote Healthy Gut Microflora: HMO prebiotic powder promotes the growth of probiotics like Bifidobacterium and Akkermansia Muciniphila.
  • Improve Immune System: HMO are more effective than traditional prebiotics (GOS, XOS, Inulin) in stimulating innate immune system, inhibiting pathogens & reducing inflammation. 
  • Support Cognitive Health: Unlike other probiotic prebiotic for adults, HMO metabolites promotes brain development, neuronal transmission, and cognition.


        Customer Reviews

        Based on 41 reviews
        Joey W.
        Pure HMO Powder

        I read a blog about this product on culturedfoodlife.com and immediately ordered. Next I listened to Beau Bergman on the Jason Wright Show. It really helped me learn so much about HMO and how it works. I intermittent fast and have a kefir shake daily so this was perfect. I’ve tried many different additions to my shakes and stick with the ones that work. This one is a keeper!

        Robert Brice
        Great products!

        Was taking another company’s HMOs before I found LO. These HMOs are just as effective at a lower price! The apple peel powder is great too! Just mix into a little bit of water at the bottom of a water bottle and swish from side to side.

        Linda Evans
        Quality product

        blends easily, does not clump

        Patricia Bergstrom
        PureHMO powder

        I love how quickly and easily this product dissolves in water and is tasteless. It makes it quick and easy to take every morning.

        Joy Langtry
        Feeling Incredible in only a week!

        I have this problem with using probiotic foods to keep my gut healthy: I start feeling really great and then get out of the habit of eating them. So, I frequently re-start using kefir on a daily basis, and did so again recently. The difference this time is that I saw Donna Schwenk (of culturedfoodlife.com) post 2 podcasts, interviewing folks about HMO products. I put a great deal of faith in Donna's recommendations, and so I tried the PureHMO from Layer Origin, and it feels like I'm getting the benefits of kefir "on steriods"!
        I've been adding a scoop to my smoothies, with greens, blueberries, kefir and Phenol powder. It's been about a week or so and I feel incredible. I have way more energy and feel good enough to do things I've been putting off out of lethargy. I am 64. I can't wait to see how I will feel after a month, and then after a year.
        If you are convinced that probiotics from food serve your health, using this product is a no-brainer in my opinion.

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        PureHMO® normalizes gut movements & blocks inflammations

        PureHMO® inhibits adhesion of pathogens & viruses to the gut lining

        Feed beneficial bacteria in the gut, like bifidobacteria to maintain a healthy gut microbiome


        Trish Whetstone with PureHMO Prebiot

        Trish Whetstone (@feelgoodgutgirl)

        Certified Health Coach +
        Master Certified Nutrition & Wellness Coach

        Trish Whetstone certification badges

        "I've been taking HMO prebiotics for over a month now, and I immediately noticed a difference in these products compared to other prebiotics ... my bowel movements have become more regular and comfortable (I am prone to constipation). I am also lactose intolerant and have had no reaction to the HMO in this."


        Scientists have identified over 200 different complex sugar molecules, known as human milk oligosaccharides (HMOs), in humans. In other mammals there are only about 50. After lactose and fats, these sugars are the third most abundant component in human milk. But humans are missing the enzymes needed to process those sugars so they pass through the stomach and into the small intestine, undigested.

        The sugars nourish a very important subspecies of bacteria called Bifidobacterium longum infantis, which then raises the acidity of the substrate as it feeds off of the HMOs.

        Inside the gut, this makes the environment more hostile to pathogens like E.Coli. The bacteria B. infantis also boosts production of proteins that close gaps between gut cells to keep microbes out of the bloodstream and increases anti-inflammatory molecules that boost the immune system. To achieve those beneficial results, B. infantis has to feed on HMOs. 


        2'-Fucosyllactose is one type of Human Milk Oligosaccharide. There are more than 200 different types of HMOs in all with 2'-FL as the most abundant variety accounting for about 30% of all HMOs. It is an oligosaccharide - specifically it is fucosylated, neutral trisaccharide composed of L-fucose, D-galactose, and D-glucose units. It can be biosynthesized at scale using E. Coli. 

        As with other oligosaccharides, a main characteristic of 2'-Fucosyllactose is its ability to protect against infectious disease. It's best known for preventing epithelial level adhesions of toxins and pathogens. The 2'-FL stimulates the growth of bifidobacteria and receptor analogons which leads to toxic and pathogenic protection. 2'-FL is known to protect against Campylobacter jejuni, Salmonella enterica serotype Typhimurium, Helicobacter pylori, and others.

        Types of Human Milk Oligosaccharides

        Research into the various health benefits of HMOs has accelerated as more HMO varieties become commercially available at scale. We're ranking benefits based on data and findings in research publications on HMOs. There is extensive researching demonstrating that 2'FL and LNnT provide benefits for gut health. There is some evidence, but not quite as strong, that both 2'FL and LNnT provide benefits for immune health. Brain health is boosted by 2'FL, 3'SL, and 6'SL according to some of the available research.

        Be sure to choose the right human milk oligosaccharide supplement for you, whether it is a 2'-FL supplement, or one that is a combination — a 2'-Fucosyllactose, Lacto-N-neotetraose supplement. 


        Both human milk oligosaccharides (HMOs) and prebiotic oligosaccharides (PBOs) belong to the large class of carbohydrates, but they have com­pletely different structures.

        Human Milk Oligosaccharides have been growing in popularity in human nutrition since 2015. HMO is naturally found in breast milk and serves as nature's preferred prebiotic. They are produced in the lactating mammary glands

        HMO is a special prebiotic that's quite different from GOS, FOS, and XOS because of where it comes from.

        Human Milk Oligosaccharide (HMO) consists of complex sugar molecules. Unlike GOS, FOS, and even XOS, which can cause some of same problems people take them to avoid (gas, bloating, IBS sypmtoms), HMO does not have that effect.

        When considering the difference be¬tween GOS/FOS and HMOs, two major aspects have to be considered:

        (A) The monosaccharide composition

        (B) The linkage between them. The linkage is very important because specific functions including defense mechanisms, immune functions, or anti-inflammatory effects strongly de-pend on those linkages.


        Research has shown that 2'-FL supports the development of a healthy gut and immune system, protection against allergic diseases and plays a role in the development of normal brain functions and cognition in early life and beyond.

        2'FL has been shown to be vital for digestive health by promoting growth of beneficial bacteria, inhibit adhesion of pathogens, quenching inflammation and normalizing gut movements.

        *HMOs primarily increase a type of intestinal microbiota called Bifidobacteria. 

        In a study of health adults charting Bifidobacteria fold change over the baseline amount, HMOs were shown to beneficially modulate the intrinsic intestinal microbiota. 

        The two HMOs tested were 2'FL and LNnT. They were compared to a placebo. 

        Want to learn more about the clinical trials? Contact us for additional information. 

        *Healthy Adults (n=100), 14 days intervention

        PureHMO Prebiotic Benefits
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